BMG’s POLARstar Omega Multi-Mode Plate Reader Performs Multiple Injections To Measure The Mitochondrial Calcium Retention Capacity

Mitochondrial dysfunction is central to the pathogenesis of acute pancreatitis as well as other diseases including ischemia-reperfusion injury of the heart, brain and kidney, muscular dystrophies and neurodegeneration.

Mitochondrial dysfunction is the result of a sudden increase in permeability of the inner mitochondrial membrane (IMM), via persistent opening of a multi-protein channel known as the mitochondrial permeability transition pore (MPTP) . This allows uncontrolled proton flow across the IMM and unregulated flux of water, ions and solutes up to 1.5 kDa into and out of the mitochondrial matrix.

This results in loss of inner mitochondrial-membrane potential, which is essential for ATP production, disruption of calcium homeostasis, swelling of mitochondria and of the outer mitochondrial membrane (OMM). Eventually cells start dying through necrosis in an uncontrolled manner.

Therefore, MPTP could be an attractive target for maintenance of mitochondrial  function and cell death prevention in a host of disease states.

You can read the app note here.

Definitive EtG/EtS LC-MS/MS Analysis: A Rugged 4-Min Method for High-Throughput Labs

Methods for monitoring alcohol consumption biomarkers EtG and EtS are generally limited by poor retention and coelution with matrix interferences, as well as by long analysis times and short column lifetimes. The dilute-and-shoot EtG/EtS LC-MS/MS analysis developed here using the novel Raptor EtG/EtS column easily resolves EtG and EtS from matrix interferences, providing consistent, accurate results for high-throughput labs testing human urine samples for alcohol consumption.

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