Although cell death is an important, normal consequence of multicellular homeostasis it also has an unfortunate role in many human diseases. Discovery of targeted cytotoxic and cytoprotective agents may therefore have important implications in treating cancer, neurodegeneration and a litany of other diseases.
In order to fully appreciate the dysregulation in a disease model, and responses to experimental stimuli, it is important establish the mechanism of action of cell death and define response kinetics. Traditional experimental approaches for addressing mode of cell death are often time consuming, labour intensive, costly and inadequate.
Here we describe the monitoring of apoptosis and necrosis in real time. Promega’s new RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay provides the simplicity of an add-mix-measure assay, in a live cell, real-time format. While the CLARIOstar with atmospheric control unit (ACU) provides the sensitive detection of duplexed luminescent and fluorescent signals, all while maintaining the appropriate [O2] and [CO2] environment for your cells.
This combination will allow you to walk away once the test is set up and ensures that you will not miss important cellular responses regardless of when changes happen.