Many cardiac drugs must be closely monitored by clinical labs to ensure that the levels in patients remain within the drug’s therapeutic range, which can be quite narrow.
A 1.8 µm fully porous particle Force Biphenyl column, along with detection by mass spectrometry, renders a fast and sensitive UHPLC assay in just 5.5 minutes. Polarity switching is used to allow the simultaneous analysis of acidic and basic compounds.
This assay includes analytes that represent the following cardiac drug classes: amiodarone (antiarrhythmic agent), digoxin (digitalis), digitoxin (digitalis), verapamil (Ca+ channel blocker), furosemide (diuretic), and flecainide (antiarrhythmic agent).
|Column||Force Biphenyl (cat.# 9629252)|
|Dimensions:||50 mm x 2.1 mm ID|
|Particle Size:||1.8 µm|
|Inj. Vol.:||2 µL|
|A:||Water + 5 mM ammonium formate + 0.1% formic acid|
|B:||Acetonitrile + 0.1% formic acid|